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KMID : 1094720000050030191
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Biotechnology and Bioprocess Engineering
2000 Volume.5 No. 3 p.191 ~ p.195
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High-performance liquid chromatographic and tandem mass spectrometric quantitation of N7 -methyldeoxyguanosine in methylated calf thymus DNA
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Chae Whi-Gun
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Abstract
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Quantitation ofN7-methyldeoxyguanosine (N7-MedG) produced in thein vitro N-methyl-N-introsourea (NMU) action on calf thymus DNA has been achieved by enzymatic degradation, liquid chromatographic separation and desorption chemical ionization tandem mass spectrometry. In conjunction with the resolving power of HPLC in the separation of isomers, desorption chemical ionization tandem mass spectrometry has been utilized in determining modified nucleosides at low levels using a stable-isotope labeled compound as an established by an independent HPLC analysis of methylated calf thymus DNA. A sensitive and specific methodology for the quantitation ofN7-MedG at the picomole level using HPLC combined with tandem mass spectrometry without radioisotope labeling process is presented. The potential of the liquid chromatographic tandem mass spectrometric analysis shows the detection ofN7-MedG as a possible marker for human exposure to methylating agentsin vitro.
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KEYWORD
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N7-methyldeoxyguanosine, N-methyl-N-nitrosourea, tandem mass spectrometry, high-performance liquid chromatography
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